Year: 2024
Type: article
Abstract: Today, biomolecular nanoparticles are prevalent as diagnostic tools and molecular delivery carriers, and it is particularly useful to examine individuals within a sample population to quantify the variations between objects and directly observe the molecular dynamics involving these objects. Using interferometric scattering as a highly sensitive label-free detection scheme, we recently developed the interferometric scattering anti-Brownian electrokinetic (ISABEL) trap to hold a single nanoparticle in solution for extended optical observation. In this perspective, we describe how we implemented this trap, how it extends the capabilities of previous ABEL traps, and how we have begun to study individual carboxysomes, a fascinating biological carbon fixation nanocompartment. By monitoring single nanocompartments for seconds to minutes in the ISABEL trap using simultaneous interferometric scattering and fluorescence spectroscopy, we have demonstrated single-compartment mass measurements, cargo-loading trends, and redox sensing inside individual particles. These experiments benefit from rich multiplexed correlative measurements utilizing both scattering and fluorescence with many exciting future capabilities within reach.
Language: English
Subfield: Atomic and Molecular Physics, and Optics
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
Awards: IMAGING OF CONFORMATIONAL CHANGES FOR SINGLE PROTEINS IN SOLUTION BY SUPPRESSION OF BROWNIAN MOTION, G222768804, EARLY CAREER: MODULATION OF LIGHT-HARVESTING BY ENDOGENOUS SWITCHES AND FUSES IN THE PHYCOBILISOME, G5648016710, IMAGING OF CONFORMATIONAL CHANGES FOR SINGLE PROTEINS IN SOLUTION BY SUPPRESSION OF BROWIAN MOTION, G8178429181
OpenAlex ID: W4404528709