FRET-based reporter assesses lysosomal DNA-degradation ability in live cells
Year: 2024
Type: article
Abstract: • A DNA-based reporter monitors lysosomal activity in live cells. • It evaluates lysosomal activity by assessing the DNA-degradation ability of lysosomes. • We successfully monitored the reduced DNA-degradation ability in dysregulated lysosomes. • We found that the lysosomal DNA-degradation ability is reduced in Niemann-pick disease cell models. Lysosomes are multifunctional organelles that serve as the cell's central hub for metabolic signaling. Lysosomal malfunction disrupts intracellular homeostasis, leading to adverse health effects. Therefore, assessing lysosomal function is vital for advancing disease understanding and guiding therapeutic development. The existing evaluation methods rely primarily on monitoring lysosomal pH and protein degradation. Here we introduce a DNA-based reporter to evaluate lysosomal activity by assessing the DNA-degradation ability of lysosomes using fluorescence imaging. We successfully monitored the lysosomal DNA-degradation ability in dysregulated lysosomes and lysosomes in drug-induced disease model of NP-A/B and NP-C. We found that both pharmacologically induced models resulted in significant reduction in lysosomal DNA-degradation ability. This tool for monitoring lysosomal activity offers valuable insights for both therapeutic development and understanding disease progression.
Source: Sensors and Actuators Reports
Authors: Jared Morse, Ka Ho Leung
Institution: Clarkson University
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,960
OpenAlex ID: W4404532185