Work

Optical diffraction tomography using a self-reference module

Year: 2024

Type: article

Abstract: Optical diffraction tomography enables label-free, 3D refractive index (RI) imaging of biological samples. We present a novel, cost-effective approach to ODT that employs a modular design incorporating a self-reference holographic capture module. This two-part system consists of an illumination module and a capture module that can be seamlessly integrated with any life-science microscope using an automated alignment protocol. The illumination module employs a galvo-scanner system, providing precise control over the angular illumination, while the capture module utilises the principle of self-reference off-axis holography. The design has a compact form factor, simple alignment, and reduced cost. Furthermore, our system offers the capability to switch between two imaging modalities, ODT and real-time synthetic aperture digital holographic microscopy (SA-DHM), a unique feature not found in other setups. Experimental results are provided using a kidney cancer cell line. Experimental results are provided using a kidney cancer cell line.

Source: Biomedical Optics Express

DOI: 10.1364/boe.545296

Authors: Zhengyuan Tang, Julianna Winnik, Bryan M. Hennelly

Institutions: National University of Ireland, Maynooth, Warsaw University of Technology

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,913

Funders: Irish Research eLibrary, Science Foundation Ireland