Work

In vivo dual-plane 3-photon microscopy: spanning the depth of the mouse neocortex

Year: 2024

Type: article

Abstract: Cortical computations arise from patterns of neuronal activity that span across all cortical layers and cell types. Three-photon excitation has extended the depth limit of in vivo imaging within the mouse brain to encompass all cortical layers. However, simultaneous three-photon imaging throughout cortical layers has yet to be demonstrated. Here, we combine non-unity magnification remote focusing with adaptive optics to achieve single-cell resolution imaging from two temporally multiplexed planes separated by up to 600 µm. This approach enables the simultaneous acquisition of neuronal activity from genetically defined cell types in any pair of cortical layers across the mouse neocortical column.

Source: Biomedical Optics Express

DOI: 10.1364/boe.544383

Authors: Matilda Cloves, Troy W. Margrie

Institutions: Sainsbury Wellcome Centre, University College London

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,913

Funders: Wellcome Trust, Gatsby Charitable Foundation