Investigating the effect of Quercetin in the presence of CoCl2 as an inducing hypoxia agent on the biological characteristics of human telomerase reverse transcription-immortalized adipose tissue-derived MSCs
Year: 2024
Type: article
Abstract: Studying the effect of small chemical molecules on stem cell characteristics under normoxia and hypoxia conditions is crucial to discovering the best conditions for effective biomedical applications. This study aimed to investigate the effect of Quercetin (QC; a flavonoid) in the presence of CoCl 2 as a mimicking hypoxia chemical on the biological features of human telomerase reverse transcription-immortalized mesenchymal stem cell (hTERT-MSC) lines. The effect of CoCl 2 , QC, and their combination on the viability, proliferation, and migration of hTERT-MSCs were evaluated by MTT, Trypan-blue staining and cell counting by hemocytometer, and in vitro wound healing assays, respectively. Moreover, the effect of treatments on the reactive oxygen species (ROS) production, cell cycle, and HIF1a , c-MET , H19 , and CASP3 gene expression was assessed by NBT, PI-staining and flow-cytometry, and real-time PCR assays, respectively. We found that CoCl 2 and QC have different effects on the viability, proliferation, and migration of hTERT-MSCs in a dose-dependent manner. In addition, CoCl 2 and QC affect ROS levels in cells in a dose- and time-dependent manner. While CoCl 2 up-regulated HIF1a , QC and CoCl 2 down-regulated CASP3 and c-MET in hTERT-MSCs. Moreover, QC reduced HIF1a and lncRNA-H19 expression in cells. Furthermore, in the presence of CoCl 2 , QC at low concentrations reduced hTERT-MSC survival, proliferation, and migration at 48 h; however, at high concentrations, it induced cell survival and proliferation. The combination treatment also up-regulated ROS levels and down-regulated the investigated genes in cells. Altogether, we conclude that QC at high concentrations under CoCl 2 -mediated hypoxia and short exposure time induces hTERT-MSCs survival and proliferation. • CoCl 2 , QC, and their combination exhibit varying effects on hTERT-MSC behaviors. • Effect of CoCl 2 , QC and their combination on cells vary with dosage and exposure time. • High dosages of QC under CoCl 2 -induced hypoxia promotes MSC survival and growth. • CoCl 2 , QC, and their combination decrease the migration of hTERT-MSCs in vitro . • CoCl 2 and QC may affect MSC behaviors by regulating HIF1a , c-MET , H19 , CASP3 , and ROS levels.
Institution: Razi University
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,990
OpenAlex ID: W4404579795