Impact of Nannochloropsis oceanica and Chlorococcum amblystomatis Extracts on UVA-Irradiated on 3D Cultured Melanoma Cells: A Proteomic Insight

Year: 2024

Type: article

Abstract: Melanoma is one of the most malignant forms of skin cancer, characterised by the highest mortality rate among affected patients. This study aims to analyse and compare the effects of lipid extracts from the microalgae Nannochloropsis oceanica (N.o.) and Chlorococcum amblystomatis (C.a.) on the intra and extracellular proteome of UVA-irradiated melanoma cells using a three-dimensional model. Proteomic analysis revealed that UVA radiation significantly increases the levels of pro-inflammatory proteins in melanoma cells. Treatment with algae extracts reduced these protein levels in both non-irradiated and irradiated cells. Furthermore, untreated cells released proteins responsible for cell growth and proliferation into the medium, a process hindered by UVA radiation through the promotion of pro-inflammatory molecules secretion. The treatment with algae extracts effectively mitigated UVA-induced alterations. Notably, UVA radiation significantly induced the formation of 4-HNE and 15-PGJ2 protein adducts in both cells and the medium, while treatment with algae extracts stimulated the formation of 4-HNE-protein adducts and reduced the level of 15-PGJ2-protein adducts. However, both algae extracts successfully prevented these UVA-induced modifications. In conclusion, lipid extracts from N.o. and C.a. appear to be promising agents in supporting anti-melanoma therapy. However, their potent protective capacity may limit their applicability, particularly following cells exposure to UVA.

Source: Cells

DOI: 10.3390/cells13231934

Authors: Agnieszka Gęgotek, Tiago Conde, Maria Rosário Domingues, Pedro Domingues, Elżbieta Skrzydlewska

Institutions: Medical University of Białystok, Centro de Estudos do Ambiente e do Mar, University of Aveiro, LAQV Requimte, Centro de Espectrometria de Massa

Language: English

Topic: Skin Protection and Aging

Subfield: Dermatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,069

Funders: Universidade de Aveiro, Rede de Química e Tecnologia, Laboratório Associado para a Química Verde, Centro de Estudos Ambientais e Marinhos, Universidade de Aveiro, Fundação para a Ciência e a Tecnologia

OpenAlex ID: W4404585162