Work

A Critical Exploration of the Total Flavonoid Content Assay for Honey

Year: 2024

Type: article

Abstract: This study critically investigates the aluminium chloride–based colorimetric determination of the total flavonoid content (TFC) of honey. Following a comprehensive review of the recent literature reporting the use of the assay in the determination of TFC in honey, 10 honeys of different botanical origins were investigated using the colorimetric method alongside an artificial honey that was used as a control. Using spiking experiments, this study demonstrates that the flavonoid concentrations commonly found in honey are too low for a direct measurement and thus some of the TFC data reported in the literature might more likely be a reflection of the honey’s inherent colour rather than a product of the coordination complex formed specifically between flavonoids and Al3+ ions. This paper highlights the importance of correct blanking and suggests alternative approaches to the traditional TFC assay for honey to ensure analysis results that are truly reflective of honey’s TFC.

Source: Methods and Protocols

DOI: 10.3390/mps7060095

Authors: Sharmin Sultana, Ivan Lozada Lawag, Lee Yong Lim, Kevin J. Foster, Cornelia Locher

Institutions: The University of Western Australia, University of the Philippines Manila, Department of Primary Industries and Regional Development

Language: English

Topic: Bee Products Chemical Analysis

Subfield: Insect Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $2,046