Work

Quantitative phase imaging with optical differentiation by spatially variable amplitude filters

Year: 2024

Type: article

Abstract: We present a non-interferometric technique for quantitative phase imaging (QPI) that is cost-effective, easily integrated into standard microscopes, and capable of wide-field imaging with noncoherent light. Our method measures the phase gradient through optical differentiation using spatially variable amplitude filters, accommodating a range of transmission functions, including commercially available variable neutral-density filters. This flexibility is made possible by a general relationship we derive. The approach is demonstrated by recovering phase information from a microsphere with a phase shift exceeding 2 π , without the need for phase unwrapping. Additionally, we image multiple HeLa cells across a large field of view.

Source: Optics Letters

DOI: 10.1364/ol.540669

Authors: Franziska Strasser, Monika Ritsch-Marte, Stefan Bernet

Institution: Institute of Biomedical Physics

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: hybrid

Funder: Austrian Science Fund

Awards: G2534911505, G7954913388