Ultrasonic Extraction of Polysaccharides from Dendrobium officinale Leaf: Kinetics, In Vitro Activities, and Characterization
Year: 2024
Type: article
Abstract: This study explored the kinetics of ultrasonic extraction of polysaccharides (DOLP) from Dendrobium officinale leaf (DOL), evaluated the in vitro bioactivity of DOL extracts and DOLP, and characterized the DOLP. A kinetic model was developed based on Fick’s second law. A technique utilizing 400 W for 50 min was employed for the ultrasonic extraction of DOLP, with an optimal solid–liquid ratio established at 1:40 (g/mL). DOL extracts dried using different methods exhibited varying antioxidant activity and inhibitory effects against α-amylase and α-glucosidase. An in vitro study revealed that DOL extracts obtained through vacuum freeze drying demonstrated significantly stronger antioxidant activity, while those derived through microwave drying showed superior inhibitory effects against α-amylase and α-glucosidase compared to the other two drying methods. Furthermore, it was observed that the in vitro bioactivity of DOLP (purity: 74.07 ± 0.52%) was significantly lower than that of DOL extracts. Nevertheless, DOLP (5.0 mg/mL) demonstrated a scavenging ability reaching 64.86% of VC for DPPH radical and 67.14% of VC for ·OH radical, and the inhibition of DOLP (10 mg/mL) on α-amylase and α-glucosidase reached 58.40% and 38.28% of the acarbose, respectively. The findings revealed that DOLP are predominantly composed of mannose, glucose, galactose, and arabinose in a distinctive molar ratio of 89.00:16.33:4.78:1.
Source: Foods
Authors: Xuerong Shi, Xuzhong Yang, Shaotong He, Ting Duan, Xin Liang, Shuzhen Ma, Jijun Gong
Institution: Central South University of Forestry and Technology
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,296
OpenAlex ID: W4404613227