A Panel of Cultures of Cancer and Normal Lung Cells with a Characterized Expression of PD-L1 to Search and Development of the Target Modifiers of Immunotherapy
Year: 2024
Type: article
Abstract: Background. The search for effective combinations of immune checkpoint inhibitors with common cytostatics, targeted cancer drugs and other treatments is a modern trend to improve the effectiveness of immunotherapy. Purpose. Development of a panel of lung cancer cell cultures and cells of normal lung tissue with a characterized molecular phenotype by expression of one of the targets of immunotherapy — programmed cell death ligand 1 (PD-L1). Methods. PD-L1 expression was quantitatively analyzed by immunofluorescence method associated with flow cytometry. Results. A panel of lung cancer cell cultures of different histotypes and cells of normal lung tissue with characterized molecular phenotype was formed according to the expression of the immunotherapy target PD-L1. In terms of PD-L1 expression intensity, cell cultures can be arranged in a series: Calu-1 HFL-1 Calu-6 Wi-26 A-549 H-596 H-211, with a 7-fold difference in the index between Calu-1 and H-211. Conclusion. The panel of cultures of cancer and normal lung cells is recommended for the search and development of effective modifiers of the immunotherapy target PD-L1.
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4404617879