Colorimetric and electrochemical analysis of DNAzyme-LAMP amplicons for the detection of Escherichia coli in food matrices
Year: 2024
Type: article
Abstract: Foodborne bacteria like Escherichia coli threaten global food security, necessitating affordable, on-site detection methods, especially in resource-limited settings. This study optimized loop-mediated isothermal amplification (LAMP) integrated with peroxidase-mimicking G-quadruplex DNA structures (DNAzyme), termed DNAzyme-LAMP which was designed to incorporate two different catalytic DNAzymes per amplification unit, enabling colorimetric detection of E. coli in leafy vegetables and milk samples. Additionally, we introduce a novel electrochemical method that enhances analytical sensitivity. The optimized DNAzyme-LAMP achieved a detection limit below 6.3 CFU per reaction or 0.1 aM gene copies. This system lays the groundwork for the development of on-site biosensors and can be adapted for detecting other foodborne pathogens.
Source: Scientific Reports
Authors: Alaa H. Sewid, Haley C. Dylewski, Joseph H. Ramos, Bailey M. Morgan, Benti D. Gelalcha, Doris H. D’Souza, Jie Jayne Wu, Oudessa Kerro Dego, Shigetoshi Eda
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,590
Funder: National Science Foundation
OpenAlex ID: W4404618751