Protein crystallization and structure determination at room temperature in the C rystal C hip
Year: 2024
Type: article
Abstract: The production of high-quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties. This protocol describes the implementation of a simple and user-friendly microfluidic device that allows both the production of crystals by the counter-diffusion method and their in situ analysis by serial crystallography. As an illustration, the whole procedure is used to determine the crystal structure of three proteins from data collected at room temperature at a synchrotron radiation source.
Source: FEBS Open Bio
Institutions: Centre National de la Recherche Scientifique, Czech Academy of Sciences, Architecture et Réactivité de l'arN, Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry, Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, Institut de Biologie Moléculaire des Plantes, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, CEA Grenoble, Institut de Biologie Structurale, Université Grenoble Alpes
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,010
Funders: Laboratoire d'Excellence NetRNA, European Commission, Agence Nationale de la Recherche, European Synchrotron Radiation Facility, Ministère de l'Europe et des Affaires Étrangères, Université de Strasbourg, Centre National de la Recherche Scientifique, Institut des sciences biologiques, NextGenerationEU
OpenAlex ID: W4404622430