Work

Structured illumination microscopy using the anti-blaze condition of a digital micromirror device

Year: 2024

Type: article

Abstract: The anti-blaze condition (ABC) is a recently introduced diffraction condition for digital micromirror devices to generate structured lights for optical metrology and imaging. Here, we present the detailed procedures and methods for applying the ABC in structured illumination microscopy (SIM). We introduce effective formulae that can be used to align the illumination optics of the ABC and a practical method to build a lookup table that can be used to find proper DMD patterns for SIM with a given objective lens. The feasibility of our proposed method is demonstrated by comparing fluorescence images obtained with and without structured lights.

Source: Optics Express

DOI: 10.1364/oe.543827

Authors: Jongwu Kim, Dug Young Kim

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,270

Funders: Korea Institute for Advancement of Technology, National Research Foundation of Korea

Awards: G4683528196, G6189580541