Work

Protocol for isolating and culturing neonatal murine cardiomyocytes

Year: 2024

Type: article

Abstract: The isolation and culture of neonatal murine cardiac cells are valuable techniques for studying their properties and molecular mechanisms in response to various treatments or conditions. Here, we present a protocol for isolating a high yield of viable neonatal murine cardiac cells, including functional, beating cardiomyocytes. We describe the steps of heart extraction, washing and pre-digestion, digestion, and cell seeding. We detail procedures for mechanical and enzymatic digestions, conducted in a controlled environment within a cell culture incubator. For complete details on the use and execution of this protocol, please refer to Bongiovanni et al. 1 • Instructions for isolating neonatal murine cardiac cells in a cell culture incubator • Steps for easy and efficient enzymatic and mechanical digestion of cardiac tissue • Guidelines for maximizing viable cardiac cell yield by stable incubator temperature • Guidelines for minimizing contamination risks through a sterile incubator environment Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The isolation and culture of neonatal murine cardiac cells are valuable techniques for studying their properties and molecular mechanisms in response to various treatments or conditions. Here, we present a protocol for isolating a high yield of viable neonatal murine cardiac cells, including functional, beating cardiomyocytes. We describe the steps of heart extraction, washing and pre-digestion, digestion, and cell seeding. We detail procedures for mechanical and enzymatic digestions, conducted in a controlled environment within a cell culture incubator.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2024.103461