High CD44 expression and enhanced E-selectin binding identified as biomarkers of chemoresistant leukemic cells in human T-ALL
Year: 2024
Type: article
Abstract: T-cell acute lymphoblastic leukemia (T-ALL) is a hematopoietic malignancy characterized by increased proliferation and incomplete maturation of T-cell progenitors, for which relapse is often of poor prognosis. To improve patient outcomes, it is critical to understand the chemoresistance mechanisms arising from cell plasticity induced by the bone marrow (BM) microenvironment. Single-cell RNA sequencing of human T-ALL cells from adipocyte-rich and adipocyte-poor BM revealed a distinct leukemic cell population defined by quiescence and high CD44 expression (Ki67neg/lowCD44high). During in vivo treatment, these cells evaded chemotherapy, and were further called Chemotherapy-resistant Leukemic Cells (CLCs). Patient sample analysis revealed Ki67neg/lowCD44high CLCs at diagnosis and during relapse, with each displaying a specific transcriptomic signature. Interestingly, CD44high expression in T-ALL Ki67neg/low CLCs was associated with E-selectin binding. Analysis of 39 human T-ALL samples revealed significantly enhanced E-selectin binding activity in relapse/refractory samples compared with drug-sensitive samples. These characteristics of chemoresistant T-ALL CLCs provide key insights for prognostic stratification and novel therapeutic options.
Source: Leukemia
Institutions: Inserm, Université Paris Cité, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Université Paris-Saclay, La Ligue Contre le Cancer, CEA Paris-Saclay - Etablissement de Fontenay-aux-roses, Stabilité Génétique, Cellules Souches et Radiations, Service Cellules Souches et Radiation, Université Côte d'Azur, Centre Méditerranéen de Médecine Moléculaire, Centre National de la Recherche Scientifique, Sorbonne Université, Institut de Biologie Paris-Seine, Institut Français de Bioinformatique, Hôpital Armand-Trousseau, Assistance Publique – Hôpitaux de Paris, Centre de Recherche Saint-Antoine, Microenvironment and B-cells: Immunopathology, Cell, Differentiation and Cancer, Université de Rennes, Centre Hospitalier Universitaire de Rennes
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,990
OpenAlex ID: W4404648000