Work

Protocol for reticulocyte enrichment from low-volume human blood samples from alpha-thalassemic and healthy participants

Year: 2024

Type: article

Abstract: Reticulocyte isolation from peripheral blood is crucial for hematological research. Here, we present a protocol for high-quality reticulocyte enrichment from small blood quantities obtained from alpha-thalassemic and healthy participants. We describe steps for Ficoll and Percoll gradient centrifugation to obtain a reticulocyte-enriched fraction, followed by negative immunomagnetic separation to remove granulocytes and platelets. This technique allows enriched reticulocyte isolation for multi-omics hematological analysis. Additionally, we detail procedures to recover peripheral blood mononuclear cells (PBMCs) and erythrocytes from the original blood sample. • Rapid reticulocyte enrichment from small blood sample • Utilizes Ficoll and Percoll for gradient centrifugation • Immunomagnetic separation for reticulocyte enrichment • Enables comprehensive hematological analysis on reticulocytes Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Reticulocyte isolation from peripheral blood is crucial for hematological research. Here, we present a protocol for high-quality reticulocyte enrichment from small blood quantities obtained from alpha-thalassemic and healthy participants. We describe steps for Ficoll and Percoll gradient centrifugation to obtain a reticulocyte-enriched fraction, followed by negative immunomagnetic separation to remove granulocytes and platelets. This technique allows enriched reticulocyte isolation for multi-omics hematological analysis. Additionally, we detail procedures to recover peripheral blood mononuclear cells (PBMCs) and erythrocytes from the original blood sample.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2024.103455