Work

The impact of bisphenol AF on skeletal muscle function and differentiation in vitro

Year: 2024

Type: article

Abstract: Various environmental chemicals have been identified as contributors to metabolic diseases. Bisphenol AF (BPAF), a substitute for bisphenol A, has been associated with changes in glucose metabolism and incidence of type 2 diabetes mellitus in humans. However, its mode of action remains unclear. Considering that skeletal muscle is the primary tissue for glucose utilization and the development of insulin resistance, yet largely neglected in toxicological assessments, we investigated the impact of BPAF on skeletal muscle function and differentiation. We examined the effects of BPAF (0.01–10 μM) on glucose uptake, response to insulin, production of reactive oxygen species (ROS), intracellular calcium, and myocyte differentiation, during hyperglycemia, insulin stimulation, and muscle contraction. We used the rat myoblast cell line L6 differentiated into myotubes, and murine primary isolated muscle fibers. In myotubes and contracting adult fibers, BPAF increased mitochondrial ROS. Basal glucose uptake was increased in myotubes while cells' ability to respond to insulin was decreased. Additionally, in developing myotubes, differentiation markers were downregulated with BPAF, along with impaired formation of tube structures. These effects were primarily observed at 10 μM concentration, which is markedly higher than reported human exposure concentrations. The results provide an insight into potential hazards associated with BPAF in terms of metabolic disruption in skeletal muscle. The developed in vitro methods show promise for future usage in assessments of new chemicals and their mixtures. • BPAF affects the function and differentiation of skeletal muscle cells. • L6 myoblast cell line and isolated muscle fibers were used as models. • BPAF increases the production of mitochondrial ROS. • BPAF increases basal glucose uptake and decreases response to insulin. • BPAF effects were observed at 10 μM concentration.

Source: Toxicology in Vitro

DOI: 10.1016/j.tiv.2024.105975

Authors: Minying Cui, Foteini Tzioufa, Joseph Bruton, Håkan Westerblad, Vesna Munic Kos

Institution: Karolinska Institutet

Language: English

Topic: Effects and risks of endocrine disrupting chemicals

Subfield: Health, Toxicology and Mutagenesis

Field: Environmental Science

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,070