Work

Antibody Data Analysis from Diverse Immune Libraries

Year: 2024

Type: preprint

Abstract: Antibody functional screening studies and next-generation sequencing require careful processing and interpretation of sequence data for optimal results. Here, we provide a detailed protocol for the functional analysis of antibody gene data, including antibody repertoire quantification and functional mapping of high-throughput screening data based on enrichment ratio values, which are a simple way to determine the enrichment of each sequenced antibody after sorting a display library against desired antigens. This protocol enables a user to apply a set of simple yet powerful bioinformatic tools for high-throughput analysis and interpretation of antibody data that is especially well suited for display library screening and for antibody discovery applications.

Source: Cold Spring Harbor Protocols

DOI: 10.1101/pdb.prot108627

Authors: Ahmed S. Fahad, Matías F. Gutiérrez-Gonzalez, Bharat Madan, Brandon J. DeKosky

Institutions: Ragon Institute of MGH, MIT and Harvard, Massachusetts Institute of Technology

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Allergy and Infectious Diseases