Work

Stationary phase effects in hydrophilic interaction liquid chromatographic separation of oligonucleotides

Year: 2024

Type: article

Abstract: > 15-mer) oligonucleotides under the mobile phase and gradient conditions evaluated here. However, several stationary phases were found to be effective for separating smaller oligonucleotides such as endonuclease digestion products. Given that early eluting oligonucleotides were found to be compatible with standard electrospray ionization conditions, several different HILIC stationary phase options are available for LC-MS studies of smaller oligonucleotides including those generated in RNA modification mapping experiments.

Source: The Analyst

DOI: 10.1039/d4an01155d

Authors: Scott Abernathy, Asif Rayhan, Patrick A. Limbach

Institution: University of Cincinnati

Language: English

Topic: Analytical Chemistry and Chromatography

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: University of Cincinnati, National Institutes of Health, National Institute of General Medical Sciences

Awards: G7022052931, G7356671099, G9000170109