Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon
Year: 2024
Type: article
Abstract: Understanding the intricate interplay between nanoparticle-mediated cyanobacterial interactions is pivotal in elucidating their impact on the transcriptome and lipidome. In the present study, total fatty acid methyl esters (FAMEs) in the wild-type (B481-WT) and transformant (B481-SD) Fremyella diplosiphon strains treated with nanoscale zero-valent iron nanoparticles (nZVIs) were characterized, and transcriptome changes analyzed. Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry revealed a 20–25% higher percentage of FAMEs in nZVI-treated F. diplosiphon strain B481-SD compared to B481-WT. Accumulation of alkanes was significantly higher (> 1.4 times) in both strains treated with 25.6 mg L −1 nZVIs compared to the untreated control. In addition, we observed significantly higher levels of monounsaturated FAMEs (11%) in B481-WT in 3.2 (11.34%) and 25.6 mg L −1 (11.22%) nZVI-treated cells when compared to the untreated control (7%). Analysis of the F. diplosiphon transcriptome treated with 3.2 mg L −1 revealed a total of 1811 and 1651 genes that were differentially expressed in B481-SD and B481-WT respectively. While the expression of iron uptake and ion channel genes was downregulated, genes coding for photosynthesis, pigment, and antioxidant enzymes were significantly ( p < 0.05) upregulated in B481-SD treated with 3.2 mg L −1 nZVIs compared to the untreated control. This study on essential FAMEs and regulation of genes in nZVI-treated F. diplosiphon strains provides a molecular framework for optimization of metabolic pathways in this model species.
Source: Scientific Reports
Language: English
Field: Energy
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $2,590
Awards: G1019936685, Excellence in Research: Oxidative stress induced impact of cell-penetrating nanoparticles on cellular constituents in a cyanobacterial model, G3937223104, National High Magnetic Field Laboratory Renewal 2023-2027, G742574937, G8152425740, ASCEND Training Model to Increase Diversity in the Biomedical Research Workforce
OpenAlex ID: W4404748731