Work

Construction of the Pseudomonas putida Strain with Low Motility and Reduced Biofilm Formation for Application in Fermentation

Year: 2024

Type: article

Abstract: Pseudomonas putida PCL1760 is a well-studied bacterium that can be used for a wide range of biotechnological applications. In our study we investigated the effect of deletion of the genes involved in alginate accumulation (algA), flagellar export (flhA), and pili formation pilQ on the behavior of the strain in bioreactors. We obtained the knockout mutant P. putida LN6160 with the deletion of these genes and showed that the absence of these genes reduces mobility and biofilm formation (40% lower after 72 h) in the mutant. At the same time, we noted the positive effect of these deletions on the growth of the mutant strain on rich medium (1.39 × 1010 CFU/mL in the mutant and 6.4 × 109 CFU/mL in the wild type) and on mineral medium (6.11 × 109 CFU/mL in the mutant and 1.36 × 109 CFU/mL in the wild type) by growing them in small-volume bioreactors. A significant decrease in the biofilm and the foam formation was also observed for LN6160 in a small-volume bioreactor. Most probably, the rapid growth of the deletion strain occurs due to a decrease in the energy load on the bacterial apparatus.

Source: Fermentation

DOI: 10.3390/fermentation10120606

Authors: Mikhail Frolov, Galim Alimzhanovich Kungurov, Emil Elmirovich Valiakhmetov, Artur Sergeyevich Gogov, Natalia Viktorovna Trachtmann, Shamil Zavdatovich Validov

Institutions: Russian Academy of Sciences, Kazan Scientific Center, A.E. Arbuzov Institute of Organic and Physical Chemistry, Kurchatov Institute

Language: English

Topic: Bacterial biofilms and quorum sensing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,955

Funder: Ministry of Science and Higher Education of the Russian Federation