Preparation, characterization, and antitumor immunity activity of polysaccharide fractions from Radix Tinosporae in vivo
Year: 2024
Type: article
Abstract: To investigate the preliminary structural characteristics and functions of polysaccharides from Radix Tinosporae, a neutral α-glucan polysaccharide, RTP-W with a molecular weight of 14.248 kDa was obtained. The potential therapeutic effect of RTP-W on hepatocellular carcinoma was investigated in vivo. Structural analysis revealed that RTP-W comprises a backbone of (1 → 4)-linked α-D-Glc p units with the occasional occupancy of α-Glc p -(1 → at the O-6 position for every nine α (1 → 4)-D-Glc p unit. Studies showed that RTP-W significantly protects the liver against cancer, extends the survival of mice with liver cancer, and lowers ALT, AST, and GGT levels in their blood. Further investigation revealed that RTP-W reversed the exhaustion of CD8 + T cells by reducing PD-1 expression and promoting cell proliferation and cytokine expression, primarily through the activation of the Akt signaling pathway. These findings indicate that RTP-W has promising potential as an immunomodulatory agent with antitumor effects.
Authors: Pingfei Li, Yongheng Zhao, Jiao Hu, Jing Chen, Yinshui Cheng, Mengnan Song, Mingqing Han, Xincai Hao, Yunfu Wang
Institutions: Hubei University of Medicine, Jingzhou Central Hospital
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,260
OpenAlex ID: W4404769102