Work

Protocol to Retrieve Unknown Flanking DNA Using Fork PCR for Genome Walking

Year: 2024

Type: article

Abstract: . The results indicated that the proposed protocol can serve as a supplement to the existing genome walking protocols. Key features • This protocol builds upon the method developed by Pan et al. [1], which is applicable to genome-walking for any species. • The developed protocol is a random priming PCR-based genome-walking scheme. • Two rounds of nested fork PCR amplifications suffice to release a positive walking result.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5161

Authors: Hongjing Wu, Hao Pan, Haixing Li

Institutions: Science and Technology College of Nanchang University, Nanchang University

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: National Natural Science Foundation of China, Jiangxi Provincial Department of Science and Technology

Awards: G1845091498, G6688124112