Anti-Inflammatory Effect of Extract from Fragaria ananassa Duch. Calyx via MAPK and NF-κB Signaling Pathway
Year: 2024
Type: article
Abstract: Currently, Fragaria ananassa Duch.are discarded as by-products except for the fruit part, so we developed a natural material using the top (= calyx), one of the by-products, and prepared an extract using 70% ethanol to investigate its effects on anti-inflammatory mechanisms.The polyphenol content of 70% ethanol extracts from Fragaria ananassa Duch.calyx was measured to be 265.86 ± 0.85 mg TAE/100 g, respectively.The antioxidant activity was confirmed through the electron donating ability and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical scavenging ability measurements.When extracts from Fragaria ananassa Duch.calyx was treated to LPSinduced RAW 264.7 cells, it was confirmed that the production of inflammation-related factors, NO, PGE 2 , iNOS, COX-2, TNF-a, and IL-6, was inhibited.In addition, it was confirmed that extracts from Fragaria ananassa Duch.calyx affected the MAPK signaling pathway by reducing the protein expression of p-ERK, p-JNK, and p-p38, which are the upper signaling pathways.In addition, it was confirmed to reduce the protein expression of p-p65 and p-IκB, which are NF-κB signaling pathways.Therefore, this study suggests that extracts from Fragaria ananassa Duch.calyx affect the regulation of the production of major inflammation-related factors by inhibiting the MAPK and NF-κB signaling pathway.These results confirmed that extracts from Fragaria ananassa Duch.calyx have the potential to be developed as a new natural material with anti-inflammatory activity.
Institutions: Kyungpook National University, Seowon University
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4404773153