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3D bioprinting of engineered exosomes secreted from M2-polarized macrophages through immunomodulatory biomaterial promotes in vivo wound healing and angiogenesis

Year: 2024
Type: article
Abstract: wound healing study. Our findings show that 3D-printed AGP hydrogel promoted M2 macrophage polarization by Janus kinase/signal transducer of activation (JAK/STAT), peroxisome proliferator-activated receptor (PPAR) signaling pathways and facilitated the M2-Exo secretion. Moreover, the COL@d-ECM/M2-Exo was found to be biocompatible with skin cells. Transcriptomic (RNA-Seq) and real-time PCR (qRT-PCR) study revealed that co-culture of fibroblast/keratinocyte/stem cells/endothelial cells in a 3D bioprinted COL@d-ECM/M2-Exo hydrogel upregulated the skin-associated signature biomarkers through various regulatory pathways during epidermis remodeling and downregulated the mitogen-activated protein kinase (MAPK) signaling pathway after 7 days. In a subcutaneous wound model, the 3D bioprinted COL@d-ECM/M2-Exo hydrogel displayed robust wound remodeling and hair follicle (HF) induction while reducing canonical pro-inflammatory activation after 14 days, presenting a viable therapeutic strategy for skin-related disorders.
DOI: 10.1016/j.bioactmat.2024.11.026
Authors: Sayan Deb Dutta, Jeong Man An, Jin Hexiu, Aayushi Randhawa, Keya Ganguly, Tejal V. Patil, Thavasyappan Thambi, Jangho Kim, Yong-kyu Lee, Ki-Taek Lim
Institutions: Kangwon National University, University of California, Davis, Hanyang University, Capital Medical University, Kyung Hee University, Chonnam National University, Korea National University of Transportation
Language: English
Subfield: Rehabilitation
Field: Medicine
Open Access status: gold
Estimated APC paid: $2,200
OpenAlex ID: W4404788076
FWCI: 7.104
Cites: 86
Cited by: 52
Related to: 10