Work

Multifunctional Nitrogen-Doped Carbon Dots to Inhibit the Aggregation of Aβ Peptide and Depolymerize the Aβ Fibrils by Modulating Reactive Oxygen Species

Year: 2024

Type: article

Abstract: Multifunctional nitrogen-doped carbon dots (N-CDs) were synthesized, and the morphology, composition and spectral properties of N-CDs were characterized by multiple characterization techniques. The inhibition of β-amyloid peptide (Aβ) aggregation and the destruction of Aβ fibril structure by N-CDs were also studied. The conformational transition and morphology of Aβ42 in the presence of N-CDs were monitored by far-UV circular dichroism (CD) spectroscopy and transmission electron microscope (TEM). The results demonstrated that the prepared N-CDs could effectively inhibit Aβ42 aggregation and depolymerize Aβ mature fibrils. Furthermore, the inhibition and disaggregation mechanism of existing Aβ42 fibrils by N-CDs was studied by electron paramagnetic resonance spectroscopy (EPR). The results showed that the modulating of reactive oxygen species (ROS) by N-CDs and multiple interactions between N-CDs and Aβ42 fibrils played a crucial part in restraining and reducing the aggregation of Aβ42. Our work demonstrates the therapeutic potential of N-CDs in suppressing Aβ42 aggregation and destroying existing Aβ42 fibrils, which provides a new perspective strategies in the treatment of AD.

Source: Langmuir

DOI: 10.1021/acs.langmuir.4c03454