Work

Optimization of culture-preservation methods to maintain developmental competence in porcine metaphase II (MII) oocytes post-in vitro maturation (IVM)

Year: 2024

Type: article

Abstract: After in vitro maturation (IVM) of porcine germinal vesicle (GV) oocytes, those that matured to the metaphase II (MII) stage were selected for further culture over a period of 24-48 h. Subsequently, these oocytes were either parthenogenetically activated or used for somatic cell nuclear transfer (SCNT) to evaluate their in vitro developmental competence. Parthenogenetically activated MII oocytes developed to the blastocyst stage after 42 h of continuous culture, whereas SCNT oocytes reached the blastocyst stage within 30 h of culture. These findings suggest that porcine MII oocytes retain their developmental competence after extended in vitro culture exceeding 30 h. This study highlights the potential of prolonged culture in enhancing the utility of MII-stage oocytes for livestock applications and possibly for future advancements in human infertility treatments.

Source: EXPERIMENTAL ANIMALS

DOI: 10.1538/expanim.24-0107

Authors: Haruhisa Tsuji, Rei Maeyama, Yoko Kato

Institutions: Asada Ladies Clinic, Kindai University

Language: English

Topic: Reproductive Biology and Fertility

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

Open Access status: diamond

Funder: Japan Society for the Promotion of Science

Award: 新規アッセイ系を用いた卵子のトランスクリプトーム解析