Molecular survey of Candida species isolated from candiduria and its risk factors in adolescent
Year: 2024
Type: article
Study design: Observational Study
Abstract: Urinary infections, especially in patients with severe diseases, are common. The present study was performed to raise awareness about the frequency of Candida ) C ) species (spp.) and evaluated risk factors of candiduria in adolescents. This cross-sectional study was conducted on 114 urine samples from patients with suspected urinary tract infections referred to the Rasoul-e-Akram Educational Hospital of Iran University of Medical Sciences (Tehran, Iran). Urine samples were examined on a microscopic slide, and for colony count and observation of co-infections, the specimens were cultured on CHROMagar Candida medium. Molecular identification of Candida at the species level was done using the PCR-sequencing approach on the internal transcribed spacer (ITS) regions. Of the 114 admitted patients, 43 cases (37.7 %) had positive for Candida spp. The typical clinical isolates were C. albicans , C. tropicalis , C. parapsilosis , and C. glabrata . The study’s survey of predisposing factors revealed that 27 (62.8 %) had an underlying disorder and malignant diseases, including pneumonia, diabetes mellitus, sepsis, and leukemia; 20.9 % of the patients were treated with cancer drugs, and 20.9 % used antibiotics. Furthermore, indwelling urethral catheters were used in over 90.7 % of patients. These results highlight the current state of candiduria in hospitalized patients and provide a solid foundation for future research in this area, inspiring further investigation and potential breakthroughs.
Source: The Microbe
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,100
Award: G82416631
OpenAlex ID: W4404803942