Semaphorin 3A repulsion directs the caudal projection of pioneer longitudinal axons in the developing chicken brain
Year: 2024
Type: article
Abstract: The medial longitudinal fasciculus (MLF) is the first axon tract to develop in the ventral vertebrate brain. It originates in the diencephalon and projects caudally into the spinal cord, pioneering the path for later developing axons. Previous anatomical and expression analyses in the chicken suggested Semaphorin 3 A (Sema3A) as the candidate to repel the amniote MLF from the forebrain. However, studies in the zebrafish implicated a distantly related semaphorin with a role in axon fasciculation, not guidance. Thus, the mechanism accounting for the caudal projection of the MLF remains unclear. Here we show that misexpression of Sema3A or grafting of Sema3A-expressing cells into the path of the MLF diverts the axons or blocks their outgrowth in chicken embryos. In vitro, Sema3A exposure resulted in the collapse of MLF growth cones. A dominant-negative approach or siRNA to interfere with the function of the Sema3A receptor Neuropilin1 allowed MLF axons to project rostrally. Together, this suggests that Sema3a repulsion directs the caudal extension of the MLF to pioneer the ventral longitudinal tract. • Sema3A is expressed rostral to the nucleus of the medial longitudinal fasciculus (MLF) prior to axon outgrowth. • Ectopic Sema3A diverts axons of the MLF. • Exposure to Sema3A causes growth cone collapse in MLF neurons. • Interfering with the semaphorin receptor Neuropilin1 allows randomisation of axon projection in theMLF. • These results indicate that Sema3A acts as a repellent to guide the MLF axons caudally.
Source: Developmental Biology
Institution: University of Portsmouth
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,690
Funders: University of Portsmouth, Royal Society
OpenAlex ID: W4404804067