Molecular basis of mRNA delivery to the bacterial ribosome
Year: 2024
Type: article
Abstract: Protein synthesis begins with the formation of a ribosome-mRNA complex. In bacteria, the 30S ribosomal subunit is recruited to many mRNAs through base pairing with the Shine Dalgarno (SD) sequence and RNA binding by ribosomal protein bS1. Translation can initiate on nascent mRNAs and RNA polymerase (RNAP) can promote recruitment of the pioneering 30S subunit. Here we examined ribosome recruitment to nascent mRNAs using cryo-EM, single-molecule fluorescence co-localization, and in-cell crosslinking mass spectrometry. We show that bS1 delivers the mRNA to the ribosome for SD duplex formation and 30S subunit activation. Additionally, bS1 mediates the stimulation of translation initiation by RNAP. Together, our work provides a mechanistic framework for how the SD duplex, ribosomal proteins and RNAP cooperate in 30S recruitment to mRNAs and establish transcription-translation coupling.
Source: Science
Institutions: John Innes Centre, Centre National de la Recherche Scientifique, Inserm, Norwich Research Park, Institut de génétique et de biologie moléculaire et cellulaire, Université de Strasbourg, University of Michigan, Technische Universität Berlin, Wellcome Centre for Cell Biology, University of Edinburgh
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Awards: G1194865751, G1399071765, G1414796561, Par-delà les frontières, l'Université de Strasbourg, G2447855386, G4460985483, G471331628, G524597515, Integrative Biology : Nuclear dynamics- Regenerative medicine - Translational medicine, G6791190511, G7002111755, G7240651115, G8089777800, G8238418921, G8337499222, G8591886698, Core Funding for the Wellcome Trust Centre for Cell Biology, Infrastructure Française pour la Biologie Structurale Intégrée, G8840145921, G8996902167
OpenAlex ID: W4404810290