Biocatalysis: modern challenges and applications
Year: 2024
Type: article
Abstract: The review analyzes recent advances, challenges, and practical applications in the field of enzymes within the framework of chemical enzymology and enzyme engineering. The achievements in the fundamental understanding of molecular mechanisms of the catalytic cycle of enzymatic reactions made using quantum mechanics/molecular mechanics methods with supercomputer technologies and bioinformatic approaches are considered. The design of protein biocatalysts with new properties is a fundamentally significant methodology of the bioengineering approach to solving practical problems, which is demonstrated by a number of examples. The increasing role of biocatalysis in medicine and biomedical research is illustrated by addressing the problems of antibiotic synthesis and overcoming antibiotic resistance of bacteria, mechanisms of neurodegenerative diseases and development of drugs to treat Alzheimer's disease, biocatalytic processes of DNA repair and the role of mechanisms of functioning of heme peroxidases in the human body. The use of enzymes to degrade endogenous and exogenous toxicants has been greatly developed in recent decades. The advances and problems of using enzymes in therapy and drug delivery are analyzed. The fundamental role of enzymes in modern analysis and diagnosis is noted. The review considers a new trend in the development of bioanalytical methods using aptamers, multi-analysis systems on biochips, surface-enhanced Raman scattering systems, and bioelectroanalysis. The bibliography includes 460 references.
Source: Russian Chemical Reviews
DOI: 10.59761/rcr5144
Authors: S. D. Varfolomeev, V. Švedas, E. N. Efremenko, A. M. Egorov, M. G. Khrenova, V. I. Tishkov, D. L. Atroshenko, A. A. Pometun, S. S. Savin, N. N. Ugarova, G. Yu. Lomakina, I. V. Gachok, I. V. Lyagin, V. I. Muronetz, A. P. Sinitsyn, O. A. Sinitsyna, A. M. Rojhkova, G. F. Makhaeva, S. O. Bachurin, O. I. Lavrik, D. O. Zharkov, A. V. Yudkina, O. M. Panasenko, A. A. Baykov, P. Masson, T. N. Pashirova, Z. M. Shaihutdinova, E. V. Popova, V. E. Tikhomirova, O. A. Kost, E. V. Kudryashova, N. V. Dobryakova, N. L. Klyachko, M. M. Veselov, A. V. Lopukhov, I. M. Le-Deygen, A. D. Usvaliev, I. V. Chudosai, N. L. Eremeev, M. I. Zvereva, M. Yu. Rubtsova, M. M. Ulyashova, G. V. Presnova, L. V. Sigolaeva, D. V. Pergushov, I. N. Kurochkin, E. G. Evtushenko, I. A. Boginskaya, J. Yu. Zvyagina, E. A. Slipchenko, O. V. Kryukova, M. V. Sedova, I. A. Ryzhikov, V. V. Shumyantseva, P. I. Koroleva, T. V. Bulko, L. E. Agafonova, R. A. Masamrekh, T. A. Filippova, A. V. Kuzikov, A. P. Savitsky, M. O. Shleeva, I. D. Solovyev, N. K. Marynich
Institutions: Russian Academy of Sciences, Lomonosov Moscow State University, Institute of Biochemical Physics NM Emanuel, Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences, Institute of Physiologically Active Compounds, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin, Federal Medical-Biological Agency, Kazan Federal University, Kazan Scientific Center, A.E. Arbuzov Institute of Organic and Physical Chemistry, Bauman Moscow State Technical University, Pirogov Russian National Research Medical University, Institute of Biomedical Chemistry
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4404826728