Global Proteomics Indicates Subcellular-Specific Anti-Ferroptotic Responses to Ionizing Radiation
Year: 2024
Type: article
Abstract: Cells have many protective mechanisms against background levels of ionizing radiation orchestrated by molecular changes in expression, post-translational modifications, and subcellular localization. Radiotherapeutic treatment in oncology attempts to overwhelm such mechanisms, but radioresistance is an ongoing challenge. Here, global subcellular proteomics combined with Bayesian modeling identified 544 differentially localized proteins in A549 cells upon 6 Gy X-ray exposure, revealing subcellular-specific changes of proteins involved in ferroptosis, an iron-dependent cell death, suggestive of potential radioresistance mechanisms. These observations were independent of expression changes, emphasizing the utility of global subcellular proteomics and the promising prospect of ferroptosis-inducing therapies for combating radioresistance.
Source: Molecular & Cellular Proteomics
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,800
Awards: G1742567106, High performance mass spectrometry: applications for the Cambridge biological sciences community, G2078231243, European Proteomics Infrastructure Consortium providing Access, G434337789, G5875395564, G6443852933, G6868616705, G6950206011, G7353250287, G7803245517, Transcription, trafficking, translation: dissecting the spatiotemporal mechanisms underlying localised protein synthesis, G831581821, G8392899497, G9000327803, Development of novel mass spectrometry approaches that enable phosphoproteomic analysis in tandem with cellular localisation
OpenAlex ID: W4404852134