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Alkamide Content and Localization in Heliopsis longipes Cypselae, Obtained via Fluorescence and Double-Multiphoton Microscopy

Year: 2024

Type: article

Abstract: The alkamide content and specific tissue localization in the cypselae of Heliopsis longipes were investigated using gas chromatography–electron ionization mass spectrometry (GC-EIMS) and multiphoton fluorescence microscopy (MPFM). GC-EIMS analysis identified two olefinic alkamides: affinin (spilanthol) and N-2-methylbutyl-2E,6Z,8E-decatrienamide. Microscopic analysis revealed that alkamides are localized within the cotyledons, and specifically compartmentalized in lipid bodies, highlighting their spatial organization. The linear unmixing of fluorescence emission fingerprints showed that affinin exhibits autofluorescence at 693 nm, corresponding to the red spectral region. This emission is attributed to the conjugated double bonds in its acyl chain. This study is the first to report on the presence and precise localization of alkamides in the cypselae of H. longipes.

Source: Molecules

DOI: 10.3390/molecules29235651

Authors: Juan Vázquez-Martínez, Jorge Molina-Torres

Institutions: Instituto Tecnológico Superior de Irapuato, Tecnológico Nacional de México, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional

Language: English

Topic: Plant chemical constituents analysis

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,069

OpenAlex ID: W4404856943

FWCI:

Cites: 23

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Related to: 10