Year: 2024
Type: article
Abstract: In this study, micropropagation protocol for the Mongolian rare shrub Lycium truncatum L. has been developed through axenical seed germination. There are successful micropropagation procedures for some Lycium species; nonetheless, L. truncatum requires both in-vitro and ex-vitro optimization.The 4-week-old sterile seedlings with spontaneous root formation, cultivated in full-strength hormone-free MS media were utilized as initial explants for in vitro culture. The highest shoot proliferation (4.6 shoots/explant) was achieved on Driver and Kuniyuki Walnut medium (DKW) supplemented with 2 mg/L kinetin (KIN) and 0.1 mg/L 1-naphtaleneacetic acid (NAA) combination. The proliferating shoots on the half-strength MS media supplemented with 0.1mg/L NAA displayed robust root formation. The rooted plantlets were acclimated in sterile soil in a pot and cultivated at room temperature for 2-3 months with a high survival rate of more than 90% before transferring to the greenhouse.
Source: The Journal of Phytology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W4404867224