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Influence of caffeic acid and Bacillus coagulans supplementation on growth, digestive enzymes, immune response, and antioxidant gene expression in common carp (Cyprinus carpio) and its resistance to Aeromonas hydrophila infection

Year: 2024

Type: article

Abstract: Present study aimed to investigate the efficacy of dietary supplementation with caffeic acid and Bacillus coagulans on growth performance, digestive enzymes, and immunity of common carp ( Cyprinus carpio ), and also fish resistance against Aeromonas hydrophila . Fish (21.0 ± 0.5 g) divided into four groups: control group (CD, commercial basal diet), caffeic acid (CA, 5 g/kg feed), B. coagulans (BC, 2×10 7 CFU/g feed), and CA+BC (5 g/kg + 2×10 7 CFU/g feed) for 8 weeks, followed by a 14-day challenge with A. hydrophila . A significant enhancement was obtained in growth performance of all treated groups compared to CD (P < 0.05). Also, feed conversion ratio was significantly reduced in treated fish compared to CD group (P < 0.05). Fish of BC and CA+BC groups revealed an increase in activity of amylase, lipase, protease, trypsin, and chymotrypsin in the intestinal tissue. In addition, amylase, lipase, protease, and trypsin in CA group exhibited a higher activity than CD (P < 0.05). A lower decrease in aspartate aminotransferase and a higher increase in alkaline phosphatas were observed in BC and CA+BC groups than CD group (P < 0.05). Values of total protein, total immunoglobulin, lysozyme activity, and alternative complement pathway (ACH50) were higher in sera of BC and CA+BC groups than CD (P < 0.05). Additionally, expression of genes related to lysozyme , IL-1β , IL-8 , TNF-α , superoxide dismutase ( SOD ), catalase ( CAT ), and glutathione peroxidase ( GPx ) in liver tissues of treatment groups were upregulated better than CD (P < 0.05). Furthermore, a significant reduction in mortality was observed in treatment groups compared to CD under the A. hydrophila challenge. These results exhibited that including a combination of caffeic acid and B. coagulans as functional dietary supplements in diet favors common carp health status and growth and, thus, can be recommended in the fish diet. • Bacillus coagulans with or without caffeic acid improved common carp growth performance. • Feeding carp B. coagulans with or without caffeic acid improved digestive enzyme activities. • Carp-fed B. coagulans with or without caffeic acid enhanced lysozyme and ACH 50 activities. • Carp-fed B. coagulans with or without caffeic acid increased survival in Aeromonas hydrophila challenge.

Source: Aquaculture Reports

DOI: 10.1016/j.aqrep.2024.102515

Authors: Seyedeh Shiva Alavinejad, Mehdi Soltani, Seyed saeed Mirzargar, Poulin Shohreh, Ali Taherimirghaed

Institutions: University of Tehran, Murdoch University, Harry Butler Institute, Amol University of Special Modern Technologies

Language: English

Topic: Aquaculture disease management and microbiota

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDGs: Zero hunger, Good health and well-being

Open Access status: gold

Estimated APC paid: $2,020