Commentary: Identification of Repeat Region Ambiguities in HLA Typing and the Implications for Immunogenetics Research
Year: 2024
Type: editorial
Abstract: Next generation sequencing (NGS) has revolutionised HLA typing, offering improvements in coverage, accuracy, resolution and throughput.Illumina and Ion Torrent platforms provide many short, but highly accurate sequence reads, while PacBio and Oxford Nanopore Technologies platforms generate fewer but much longer sequence reads.The lower raw accuracy of long reads is mitigated by consensus sequence generation and crucially allows phasing of polymorphisms across the entire lengths of the HLA genes.Combinations of short and long read sequencing technologies are also in use, utilising the advantages of both techniques to further improve accuracy.Together, NGS technologies are driving rapid increases in the numbers of alleles, many as full-length genomic sequences, submitted to the IPD-IMGT/HLA Database.Submissions from hitherto less well characterised HLA genes has also grown, enriching the database as a whole.
Source: HLA
DOI: 10.1111/tan.15768
Authors: Thomas R. Turner, Richard H. L. Natarajan, James Robinson, Steven G. E. Marsh, Neema P. Mayor
Institutions: Anthony Nolan, The Royal Free Hospital, University College London
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,550
OpenAlex ID: W4404890110