Work

Shining a Light on Spectrophotometry in Bacteriology

Year: 2024

Type: article

Abstract: Spectrophotometry is widely used in biological sciences. In bacteriology, spectrophotometric monitoring of cell numbers during cultivation provides a rapid assessment of growth. Unfortunately, familiarity with this technique has led scientists to become complacent in its usage. Here, we review some guiding principles of spectrophotometry and practical considerations that may influence the outcome of experiments. This perspective is intended to assist both new and seasoned scientists in presenting robust and reproducible growth data based on spectrophotometric readings.

Source: Antibiotics

DOI: 10.3390/antibiotics13121164

Authors: Veronica Montesinos-Cruz, Greg A. Somerville

Institution: University of Nebraska–Lincoln

Language: English

Topic: Bacterial Identification and Susceptibility Testing

Subfield: Clinical Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,296

OpenAlex ID: W4404939606

FWCI: 1.186

Cites: 20

Cited by: 5

Related to: 10