Dynamic alterations in bacterial and fungal microbiome and inflammatory cytokines following SRV-8 infection in cynomolgus monkeys
Year: 2024
Type: article
Abstract: While viral infections can disturb the host gut microbiome, the dynamic alterations in microbial composition following infection remain poorly characterized. This study identified SRV-8-infected monkeys and classified them into five groups based on infection progression. 16S rRNA amplicon sequencing revealed significant alterations in the relative and inferred absolute abundance of bacterial genera UCG-002, Agathobacter, Coprococcus, and Holdemanella during the early stage of SRV-8 infection, coinciding with provirus formation. These microbial shifts were accompanied by functional modifications in bacterial communities at the same stage. In contrast, ITS amplicon sequencing indicated no significant differences in fungal composition between healthy wild-type and SRV-8-infected monkeys. Spearman correlation analyses demonstrated close interactions between intestinal bacteria and fungi following SRV-8 infection. Additionally, SRV-8 seropositive groups exhibited significantly elevated mRNA expression levels of pro-inflammatory (TNF-α, IFN-γ, IL-1β, and IL-6) and anti-inflammatory (IL-10) cytokine genes, highlighting close associations between inflammatory cytokines and immune responses. Overall, these findings provide a comprehensive characterization of bacterial and fungal microbiota dynamics and inflammatory cytokine responses associated with SRV-8 infection, clarifying the pathobiological mechanisms underlying SRV-8 infection from the perspective of the gut microbiome.
Source: 动物学研究
Authors: Yang Yun-Peng, 扬州大学兽医学院江苏高校动物重要疫病与人兽共患病防控协同创新中心, 江苏 扬州 225009, 中国, Xu Li-Bing, Lu Yong, Wang Jing, Nie Yan-Hong, Sun Qiang, 扬州大学比较医学研究院, 江苏 扬州 225009, 中国, 上海脑科学与类脑研究中心, 上海 201602, 中国, 中国科学院神经科学研究所中国科学院灵长类神经生物学重点实验室, 神经科学国家重点实验室, 中国科学院脑科学与智能技术卓越创新中心, 上海 200031, 中国, 中国科学院昆明动物研究所遗传进化与动物模型全国重点实验室, 云南 昆明 650201, 中国
Language: English
Topic: Gut microbiota and health
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4404945653