Work

Derivation of two induced pluripotent stem cell lines from a healthy control subject

Year: 2024

Type: article

Abstract: Two human induced pluripotent stem cell lines, LEIi021-A and LEIi021-B, were derived from dermal fibroblasts from a healthy control subject from an Australian Aboriginal family with retinitis pigmentosa-11. Reprogramming was performed using episomal vectors expressing OCT4, SOX2, LIN28, L-MYC, KLF4 and mp53DD. Pluripotency markers were expressed in both LEIi021-A and LEIi021-B lines. The two cell lines displayed normal karyotypes and demonstrated the ability to differentiate into embryoid bodies with the three primary germ layers and retinal pigment epithelial cells.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2024.103621

Authors: Dan Zhang, Di Huang, Leon M. Larcher, Khine Zaw, Shang-Chih Chen, Luke Jennings, Tina M. Lamey, Jennifer A. Thompson, Terri L. McLaren, Fred K. Chen, Samuel McLenachan

Institutions: Lions Eye Institute, Sir Charles Gairdner Hospital, The University of Western Australia, Royal Perth Hospital, Perth Children's Hospital

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,320

Funders: Channel 7 Telethon Trust, Retina Australia, McCusker Charitable Foundation, National Health and Medical Research Council, Department of Health, Government of Western Australia, Western Australian Future Health and Innovation Fund, Government of Western Australia

Awards: Accelerating the identification and treatment of splice-altering mutations underlying inherited retinal diseases, G7276399713

OpenAlex ID: W4404958160

FWCI: 0.115

Cites: 6

Cited by: 1

Related to: 10