Mycobacteria Treatment Inhibits Bladder Cancer Cell Migration, Invasion, and Anchorage-Independent Growth
Year: 2024
Type: article
Abstract: Bladder cancer (BC) is a highly recurrent and invasive malignancy, with Mycobacterium bovis BCG serving as the primary immunotherapy, particularly for non-muscle-invasive bladder cancer (NMIBC). However, the mechanisms underlying BCG’s antitumor effects and the potential of non-tuberculous mycobacteria like Mycobacterium brumae remain unclear. This study investigates the antitumor effects of M. bovis BCG and M. brumae on BC cell migration, invasion, and anchorage-independent growth. BC cell lines representing different stages of tumor differentiation were treated with either M. bovis BCG or M. brumae. Cell migration was assessed through wound healing and transwell assays, invasiveness by transwell invasion assays, MMP-9 production by gelatin zymography, and anchorage-independent growth via soft agar colony formation. Both mycobacteria inhibited individual cell migration across all BC lines, while collective migration was only reduced in intermediate-grade cells. Both treatments also reduced invasiveness, associated with decreased MMP-9 production. Furthermore, M. brumae inhibited anchorage-independent growth across all BC lines, while M. bovis BCG had a more selective effect, primarily inhibiting growth in high-grade cells. In conclusion, both mycobacteria reduce migration, invasion, and anchorage-independent growth of BC cells, with their effectiveness varying by species and tumor differentiation grade.
Institution: Universitat Autònoma de Barcelona
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,296
Awards: G1044684712, G1105844351, G1145731905, G1175115578, G1392280148, G1455481585, G148081399, G1858345700, G1866028767, G210041235, G2262748287, G2300471596, G2390397592, G2601397997, G284665130, G300979063, G3320050967, G34346518, G3480869486, G3511743623, G3681454997, G4042783231, G4209496302, G425668148, G4421430368, G4633479443, G491712853, G5045040751, G5080475149, G5195188938, G5404395142, G5682652210, G586857820, G624877701, G6731524417, G6998210420, G7084143925, G7805626310, G7863371703, G8497050386, G8627740392
OpenAlex ID: W4404962868