Work

Rolling Circle Amplification Integrating with Exonuclease-III-Assisted Color Reaction for Sensitive Telomerase Activity Analysis

Year: 2024

Type: article

Abstract: Telomerase activation can lead to the escape from cell senescence and immortalization, playing a crucial role in the growth and proliferation of cancer cells. Therefore, the detection of telomerase activity is essential for cancer diagnosis and treatment. Herein, we develop a novel ultrasensitive and visually detectable platform. By incorporation of exonuclease-III (Exo-III), this platform achieves dual signal amplification of rolling circle amplification products. Additionally, the colorimetric analysis of 3,3',5,5'-tetramethylbiphenyl (TMB) chromogenic reaction system provides this approach with unique advantages such as simplicity, speediness, and sensitivity. The detection platform exhibits high sensitivity and specificity in actual sample testing, which aligns closely with results obtained using commercial kits. Moreover, it offers ease-of-use through visual determination by the naked eyes. This finding indicates that our proposed sensing method performs satisfactorily in detecting telomerase in real biological samples. Henceforth, we believe that this sensing platform holds great potential for clinical diagnosis and anticancer drug development.

Source: ACS Omega

DOI: 10.1021/acsomega.4c03839

Authors: Xiaoya Liu, Xianxian Zhao, Jie Zhang, Yihan Wang, Xiaoping Ye

Institutions: The Affiliated Yongchuan Hospital of Chongqing Medical University, Chongqing University, Fuling Center Hospital of Chongqing

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,935