Figure 2 from Single-Cell Analysis of Bone Marrow CD8+ T Cells in Myeloid Neoplasms Reveals Pathways Associated with Disease Progression and Response to Treatment with Azacitidine
Year: 2024
Type: other
Abstract: Identification of a CD8+ subpopulation (CD57+CXCR3+) which distinguishes patients with MDS from patients with AML and CMML. A, Representative viSNE plots, derived from the FlowSOM analysis of BM CD8+ T cells from patients with LR-MDS (n = 12), HR-MDS (n = 15), AML (n = 16), and CMML (n = 5). B, Bar plots displaying the proportion of the metaclusters between the groups, expressed as percentage within CD8+ T cells. C, Heatmap depicting the expression level of the T-related markers between the metaclusters. D, Violin plots showing the expression level of CXCR3 in metacluster 1. E, Representative flow cytometry plots for the identification of the CD57+CXCR3+CD8+ T cell subpopulation in a cohort of patients with LR-MDS (n = 7), HR-MDS (n = 27), AML (n = 20), and CMML (n = 10). F, Percentage of CD57+CXCR3+ cells within CD8+ T cells. Kruskal–Wallis was used in B and D. One-way ANOVA followed by the “two-stage” Benjamini, Krieger, and Yekutieli multiple comparison test was used in F. *, P < 0.05; ***, P < 0.001.
Authors: Athanasios Tasis, Nikos E. Papaioannou, Maria Grigoriou, Nikolaos Paschalidis, Catherine Loukogiannaki, Anastasia Filia, Kyriaki Katsiki, Eleftheria Lamprianidou, Vasileios Papadopoulos, Christina Maria Rimpa, Antonios Chatzigeorgiou, Ioannis Kourtzelis, Petroula Gerasimou, Ioannis Kyprianou, Paul Costeas, Panagiotis Liakopoulos, Konstantinos Liapis, Petros Kolovos, Triantafyllos Chavakis, Themis Alissafi, Ioannis Kotsianidis, Ioannis Mitroulis
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4405002437