Figure 5 from Single-Cell Analysis of Bone Marrow CD8+ T Cells in Myeloid Neoplasms Reveals Pathways Associated with Disease Progression and Response to Treatment with Azacitidine
Year: 2024
Type: other
Abstract: BM-derived CD8+ T cells from responders (CR) to AZA show an enhanced ISG molecular signature compared with nonresponders (FAIL) in scRNA-seq analysis. A, Comparison of separate UMAPs for CR (a total of 13,667 cells, including 7,571 from patients with MDS and 6,096 cells from patients with secondary AML, respectively) and FAIL patients (a total of 14,782 cells, including 8,026 cells from patients with MDS and 6,756 cells from patients with secondary AML, respectively). B, Stacked bar chart showing the average distribution of clusters between the two groups. The percentage of cluster 10 (proliferative) was increased in CR compared with FAIL patients (unpaired Student t test, P = 0.0418). C, Dot plot representing MSigDB (Hallmark 2020) enrichment analysis of positively enriched pathways in CR patients. Enriched pathways with a q-value <0.05 (Benjamini–Hochberg correction) are shown. D, Gradient expression of representative selected genes involved in IFN-related pathways, as they are projected onto UMAPs. E, ISG score of cluster 0 (GZMK) and violin plots showing the expression levels of the top differentially expressed IFN-stimulated genes of cluster 0 (GZMK) between CR and FAIL. F, ISG score of cluster 1 (EOMES) and violin plots showing the expression levels of the top differentially expressed IFN-stimulated genes of cluster 1 (EOMES) between CR and FAIL. G, ISG score of cluster 2 (CTL_1) and violin plots displaying the expression levels of the top differentially expressed IFN-stimulated genes of cluster 2 (CTL_1) between CR and FAIL. H, ISG score of cluster 4 (CTL_2) and violin plots displaying the expression levels of the top differentially expressed IFN-related genes of cluster 4 (CTL_2) between CR and FAIL.
Authors: Athanasios Tasis, Nikos E. Papaioannou, Maria Grigoriou, Nikolaos Paschalidis, Catherine Loukogiannaki, Anastasia Filia, Kyriaki Katsiki, Eleftheria Lamprianidou, Vasileios Papadopoulos, Christina Maria Rimpa, Antonios Chatzigeorgiou, Ioannis Kourtzelis, Petroula Gerasimou, Ioannis Kyprianou, Paul Costeas, Panagiotis Liakopoulos, Konstantinos Liapis, Petros Kolovos, Triantafyllos Chavakis, Themis Alissafi, Ioannis Kotsianidis, Ioannis Mitroulis
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4405002474