Work

Effect of Temperature and Inoculum-to-Substrate Ratios on Two-Stage Biohydrogen and Methane Production from Sugarcane Molasses

Year: 2024
Type: article
Abstract: The effect of temperature (37 and 55 °C) and inoculum-to-substrate ratios (ISRs) on biohydrogen (first-stage ISRs 0.25, 0.5, and 1) and methane (second-stage ISRs 2, 4, and 6) production during two-stage anaerobic digestion of molasses using mixed anaerobic consortia was investigated. At 37 °C, ISRs 1 and 4 produced the highest yields of biohydrogen (63.61 NL-H 2 /kg-VS added ) (VS: volatile solid) and methane (1092.70 NL-CH 4 /kg-VS added ). At 55 °C, ISR 0.5 yielded 25.03 NL-H 2 /kg-VS added, while ISR 4 yielded 806.54 NL-CH 4 /kg-VS added . Firmicutes, specifically Clostridia, dominated the first stage at both temperatures. The second stage (ISR 4) at 37 °C included 16.06% Chloroflexi and 22.84% Firmicutes, while at 55 °C, it included 7.55% Thermotogota.
Language: English
Open Access status: green
OpenAlex ID: W4405013227
FWCI: 0.561
Cites: 106
Cited by: 3
Related to: 10