Identification Methods of Tumor Tissue Origin Based on Different STR Typing Kits.
Year: 2024
Type: article
Abstract: OBJECTIVES: To establish the identification method of tumor tissue origin based on commonly used STR typing kits. METHODS: ) and identity by state (IBS) scores were calculated within the paired carcinoma (PC), the tumor-unrelated individual (UI), the tumor-simulated full sibling (FS) and the tumor-simulated parent-offspring (PO) groups. The tumor tissue origin identification and prediction models of 8 commonly used STR typing kits were established based on the statistical results obtained above, and an attempt was made to establish a specific model for tumor tissue origin identification. The accuracy, sensitivity and specificity were verified and evaluated using the additional 23 paired tumor tissue samples. RESULTS: ) in UI group were significantly lower than those of other STR loci. (3) The tumor tissue origin identification and prediction models of 8 commonly used STR typing kits and the identification model of tumor tissue origin with 15 STR loci (15-STRs) were successfully established, with sensitivity of 100%, specificity of 97.56%-99.88%, and accuracy of 97.59%-99.89%. Among them, the 15-STRs model had 100% sensitivity, 99.88% specificity, and 99.89% accuracy, which were higher than those of commonly used commercial kits. CONCLUSIONS: This study successfully establishes the tumor tissue origin identification methods with 8 commonly used STR typing kits, which expands the application of tumor tissue origin identification. In addition, the differences of different loci in the identification of tumor tissue origin were compared, and 15 STR loci which were particularly suitable for the identification of tumor tissue origin were selected, providing the data basis for the establishment of tumor origin tracing kits in future.
Source: PubMed
Authors: Li-Ming Wu, An-Qi Chen, Su-Hua Zhang, Cheng-Tao Li
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4405028585