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Application of Protease-Hydrogen Peroxide Digestion Method in Forensic Diatom Examination.

Year: 2024
Type: article
Abstract: OBJECTIVES: To propose a novel forensic diatom examination method, namely the protease-hydrogen peroxide (PHP) digestion method, and to explore its potential application in forensic practice. METHODS: The PHP digestion method was applied to process lung tissues and compared with the traditional nitric acid digestion method in terms of diatom quantity, recovery rate, proportion of diatom fragments, and recovery efficiency of different diatom genera. RESULTS: <0.05). At the same time, the diatom recovery stability of the PHP digestion method was higher than that of the nitric acid digestion method. CONCLUSIONS: The PHP digestion method is superior to the traditional nitric acid digestion method in the lung tissue diatom extraction efficiency. Additionally, it holds significant advantages in safety, environmental protection, and other aspects. It is expected to be applied in forensic drowning identification.
Source: PubMed
Language: English
Field: Medicine
Open Access status: green
OpenAlex ID: W4405028717
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