[Tougu Xiaotong Capsule alleviates cartilage degeneration in mice with knee osteoarthritis by modulating Nav1.7].
Year: 2024
Type: other
Abstract: OBJECTIVE: Capsule (TGXTC) alleviates chondrocyte degeneration in knee osteoarthritis (KOA). METHODS: hybridization (FISH) was used to detect Nav1.7 expression in the chondrocytes. In cultured KOA chondrocytes, the effect of TGXTC and lentivirus-mediated Nav1.7 knockdown on MMP-3, MMP-13, ADAMTS-4, ADAMTS-5, and COX-2 protein expressions were assessed with Western blotting. RESULTS: In KOA mice treatments with TGXTC and diclofenac sodium both significantly alleviated structural damage of the cartilage layer, reduced Nav1.7 protein and mRNA expressions and lowered the expressions of MMP-3, ADAMTS-5, and COX-2 proteins in the cartilage tissues. FISH results indicated that TGXTC treatment significantly reduced IL-1β -induced Nav1.7 expression in the chondrocytes. In Nav1.7 knockdown experiment, Nav1.7 levels were significantly lower in IL-1β+sh-Nav1.7 group than in IL-1β group, and also lower in IL-1β+TGXTC group than in IL-1β+sh-Nav1.7+TGXTC group. TGXTC treatment significantly inhibited IL-1β-induced elevation of MMP-3, MMP-13, ADAMTS-4, ADAMTS-5 and COX-2 protein expressions in the chondrocytes, but its effects were strongly weakened by Nav1.7 knockdown. CONCLUSION: TGXTC alleviates extracellular matrix metabolic disorder in KOA chondrocytes by regulating Nav1.7, thereby mitigating chondrocyte degeneration in KOA mice.
Source: PubMed
Institution: Fujian University of Traditional Chinese Medicine
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4405028836