Work

Blocking of Tim‐3 Ameliorates Spinal Cord Ischemia‐Reperfusion Injury Through Inhibiting Neuroinflammation and Promoting M1‐to‐M2 Phenotypic Polarization of Microglia

Year: 2024

Type: article

Abstract: BACKGROUND: Blocking of Tim-3 exerts therapeutic effects in a series of ischemia-reperfusion injury (IRI). METHODS: In this work, a cross-clamped aortic arch was conducted to establish SCIRI rat model. Besides, rat spinal microglia was subjected to OGD/R to mimic I/R-like conditions in vitro. The in vivo and in vitro therapeutic effects of Tim-3 antibody in SCIRI were investigated from these aspects: neuronal apoptosis, neuroinflammation, microglia activation, and polarization. RESULTS: It was verified that Tim-3 was highly expressed in spinal cord tissues of SCIRI rats and blocking of Tim-3 attenuated SCIRI-induced pathological injury, neuronal apoptosis, neuroinflammation, and microglia activation (M1 polarization). In addition, it was verified that Tim-3 was highly expressed in OGD/R-treated rat spinal microglia and blocking of Tim-3 attenuated OGD/R-induced inflammation and spinal microglia activation (M1 polarization). CONCLUSIONS: Tim-3 antibody can exert therapeutic effects in SCIRI through inhibiting neuroinflammation and promoting microglia polarization from M1 to M2 phenotype.

Source: Immunity Inflammation and Disease

DOI: 10.1002/iid3.70084

Authors: Zhenxing Li, Binbin Zhou, Guanghui Chen, Xiangyu Yang, Han Su, Bolin Li

Institutions: The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Guangxi University of Chinese Medicine

Language: English

Topic: Neuroinflammation and Neurodegeneration Mechanisms

Subfield: Neurology

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,240