Description of Anaerococcus kampingiae sp. nov., Anaerococcus groningensis sp. nov., Anaerococcus martiniensis sp. nov., and Anaerococcus cruorum sp. nov., isolated from human clinical specimens
Year: 2024
Type: article
Abstract: OBJECTIVES: To improve the identification of anaerobic bacteria, the identity of clinical isolates which could not be identified using MALDI-TOF MS was assessed using whole genome sequencing (WGS) and in-house made main spectral profiles (MSPs) were created. Four novel Anaerococcus species, each represented by at least two isolates, were encountered. METHODS: The novelty of the isolates was confirmed by comparing the 16S rRNA gene sequences and the WGS with their closest relatives. Phylogenetic and phylogenomic relationships were determined using MEGA X and DSMZ TYGS. Biochemical features were determined and the clustering of the created MSPs was calculated. Possible clinical relevance was assessed. RESULTS: The novelty of the four different species was confirmed by the ANI value, and phylogenetic/phylogenomic clustering. Three of these species shared the same biochemical features, while one showed a different pattern. Only this latter species can be differentiated from other Anaerococcus spp. Remarkebly, six of the ten isolates were obtained from a positive blood culture, of which in five cases the bacterium was the only species encountered. CONCLUSIONS: (accession numbers PP192778/JBGMEH000000000)).
Source: Anaerobe
Authors: K.E. Boiten, J. Meijer, E.M. van Wezel, A.C.M. Veloo
Institutions: University Medical Center Groningen, University of Groningen
Language: English
Subfield: Clinical Biochemistry
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,200
OpenAlex ID: W4405212239