Year: 2024
Type: article
Abstract: Blood coagulation is a highly regulated injury response that features polymerization of fibrin fibers to prevent the passage of blood from a damaged vascular endothelium. A growing body of research seeks to monitor coagulation in microfluidic systems but fails to capture coagulation as a response to disruption of the vascular endothelium. Here we present a device that allows compression injury of a defined segment of a microfluidic vascular endothelium and the assessment of coagulation at the injury site. This pressure injury-on-a-chip (PINCH) device allows visualization of coagulation as the accumulation of fluorescent fibrin at injury sites. Quantification of fluorescent fibrin levels upstream of and at injury sites confirm that pre-treating vascular endothelium with fluid shear stress helps capture coagulation as an injury response. We leverage the PINCH devices to demonstrate the limited coagulation response of type A hemophiliacs and evaluate the performance of hemostatic microparticles and fibrinolytic nanoparticles. Our findings and the straightforward fabrication of the PINCH devices make it a promising choice for additional screening of hemostatic therapeutics.
Source: Lab on a Chip
DOI: 10.1039/d4lc00471j
Authors: Halston Deal, Elizabeth M. Byrnes, Sanika Pandit, Anastasia Sheridan, Ashley C. Brown, Michael Daniele
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Awards: CAREER: Reconfigurable Microfluidic-Microbalance Sensors to Monitor and Optimize the Performance of Microphysiological Models, G2492084534, Microphysiological Models to Evaluate the Role of Age-Dependent Fibrinogen Sialylation in Wound Healing, CAREER: Dynamic Microgels that Mimic Platelet Behavior to Promote Healing, NC State Molecular Biotechnology Training Program (MBTP), Training Grant in Comparative Molecular Medicine
OpenAlex ID: W4405219940